Molecular Diagnostics

Focuses on the development and evaluation of new molecular-based approaches for rapidly and accurately assessing biological samples for the presence of specific pathogens as well as other microorganisms of interest.

The ‘Molecular diagnostics’ research program focuses on the development and evaluation of new molecular-based approaches for rapidly and accurately assessing biological samples for the presence of specific pathogens as well as other microorganisms of interest. More specifically, molecular assays based on Polymerase Chain Reaction (PCR), real-time PCR and DNA arrays are being developed and exploited for fast and reliable (multiplex) detection, identification, and quantification of target organisms. These techniques are then further adapted for high-throughput analyses in practical laboratory settings as well as for epidemiological and ecological studies.

New developments are done in close collaboration with PME&BIM.


 

Research and consulting - topics

  • Development of new DNA- and RNA-based diagnostic tools for rapid microbial detection and identification
  • Development of DNA arrays to simultaneously detect an expanding set of microorganisms (e.g. DNA Multiscan® for plant diseases and beneficials) 
  • Development of specific (real-time) PCR assays
  • Phenotypic and genotypic characterization of microbial strains isolated from various matrices
Contact
Stefan Van Kerckhove

Research Associate
Scientia Terrae vzw

svk@scientia.be

Publications
Diagnosemembranen voor snelle detectie en identificatie van bodemgebonden schimmels.

Lievens, B. 2003.
Verbondsnieuws 1: 42-43. 

Assessing populations of a disease suppressive microorganism and plant pathogen using DNA arrays.

Lievens, B., Claes, L., Vanachter, A.C.R.C., Krause, M.S., Cammue, B.P.A., Thomma, B.P.H.J. 2007.
Plant Science 172: 505-515.

To download these publications you'll need to login
login or request an account.

A robust identification and detection assay to discriminate the cucumber pathogens Fusarium oxysporum f. sp. cucumerinum and f. sp. radicis-cucumerinum.

Lievens, B., Claes, L., Vakalounakis, D.J., Vanachter, A.C.R.C., Thomma, B.P.H.J. 2007.
Environmental Microbiology 9: 2145–2161.

To download these publications you'll need to login
login or request an account.

Development of a DNA array for early detection and rapid identification of tomato vascular wilt pathogens.

Lievens, B., Brouwer, M., Cammue, B.P.A., De Ceuster, T.J.J., Vanachter, A.C.R.C., Thomma, B.P.H.J. 2003.
Communications in Applied Biological Sciences 68: 569-582

Diagnosemembranen voor detectie en identificatie van bodemschimmels.

Lievens, B. 2002.
Proeftuinnieuws 23: 44-45.

Horticulture becomes 'high tech'. Multiplex detection and identification of plant pathogens by DNA arrays.

Lievens, B., Brouwer, M., Vanachter, A. C. R. C., Cammue, B. P. A., Thomma, B.P.H.J. 2004.
ISHS Acta Horticulturae 698.

Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum.

Lievens, B., Rep, M., Thomma, B.P.H.J. 2008.
Pest Management Science 64:781-788.

To download these publications you'll need to login
login or request an account.

Schadelijke Fusarium in komkommer opsporen via DNA-analyse.

Lievens, B. 2007.
Proeftuinnieuws 8: 33-34.

Plantenziekten binnen 36 uur ontdekt met DNA Multiscan®.

Lievens, B. 2005.
Proeftuinnieuws 5: 20-21.

Detecting single nucleotide polymorphisms using DNA arrays for plant pathogen diagnosis.

Lievens, B., Claes, L., Vanachter, A.C.R.C., Cammue, B.P.A., Thomma, B.P.H.J. 2006.
FEMS Microbiology Letters 255: 129-139.

To download these publications you'll need to login
login or request an account.

Recent developments in pathogen detection arrays: implications for fungal plant pathogens and use in practice.

Lievens, B., Thomma, B. P. H. J. 2005.
Phytopathology 95:1374-1380.

To download these publications you'll need to login
login or request an account.

Recent developments in diagnostics of plant pathogens: a review.

Lievens, B., Grauwet, T. J. M. A., Cammue, B. P. A., Thomma., B. P. H. J. 2005.
Recent Research Developments in Microbiology 9: 57-79.

To download these publications you'll need to login
login or request an account.

Quantitative assessment of phytopathogenic fungi in various substrates using a DNA macroarray.

Lievens, B., Brouwer, M., Vanachter, A.C.R.C., Lévesque, C.A., Cammue, B.P.A., Thomma, B.P.H.J. 2005.
Environmental Microbiology 7:1698-1710.

To download these publications you'll need to login
login or request an account.

Quantification in multiplex format as a challenging goal for plant pathogen molecular diagnostic assays.

Lievens, B., Thomma, B.P.H.J. 2007.
Pest Technology 1: 17-26 (Invited review).

To download these publications you'll need to login
login or request an account.

Quantifcation of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR.

Brouwer, M., Lievens, B., Van Hemelrijck, W., Van den Ackerveken, G., Cammue, B.P.A., Thomma, B.P.H.J. 2003. 
FEMS Microbiology Letters 228: 241-248.

To download these publications you'll need to login
login or request an account.

The presence of a virulence locus discriminates Fusarium oxysporum isolates causing tomato wilt from other isolates.

Van der Does, H. C., Lievens, B., Claes, L., Houterman, P. M., Cornelissen, B. J. C., and Rep, M. 2008.
Environ. Microbiol. 10: 1475-1485.

To download these publications you'll need to login
login or request an account.

Effector gene screening allows unambiguous identification of Fusarium oxysporum f. sp. lycopersici races and discrimination from other formae speciales.

Lievens, B., Houterman, P. M. and Rep, M. 2009.
FEMS Microbiol. Lett. 300: 201-215.

To download these publications you'll need to login
login or request an account.

Evolutionary relationships between Fusarium oxysporum f. sp. lycopersici and F. oxysporum f. sp. radicis-lycopersici isolates inferred from mating type, elongation factor-1α and exopolygalacturonase sequences.

Lievens, B., Van Baarlen, P., Verreth, C., Van Kerckhove, S., Rep, M. and Thomma, B. P. H. J. 2009. 
Mycol. Res. 113:1181-1191.

To download these publications you'll need to login
login or request an account.

Get in touch

Interested in our service? Contact us for a detailed quote on products or on custom advice.

Scientia Terrae

Fortsesteenweg 30A
B-2860 St.-Katelijne-Waver
Belgium

contact@scientia.be

+32(0)15 30 55 90
+32(0)15 30 55 99

Research institute

About

Staff

Jobs

Publications

Contact

© 2019 Scientia Terrae Research Institute


Protected with reCAPTCHA
Privacy - General conditions